DSpace Repository

In Vitro Antiproliferative Activity Of Amaryllidaceae Species Against The K562 Human Leukaemia Cell Line

Show simple item record

dc.contributor.author Napo, Kgaogelo, Vincent
dc.contributor.author Mokoena, Lebohang, Unice
dc.contributor.author Mangoejane, Charlott
dc.contributor.author Mfengwana, Hilda
dc.contributor.author Mashele, Samson
dc.contributor.author Sekhoacha, Mamello, Patience
dc.date.accessioned 2023-05-08T05:58:16Z
dc.date.available 2023-05-08T05:58:16Z
dc.date.issued 2020-10-12
dc.identifier.issn 0254-6299
dc.identifier.other https://doi.org/10.1016/j.sajb.2020.07.012
dc.identifier.uri http://hdl.handle.net/11462/2455
dc.description Article en_US
dc.description.abstract Acute lymphocytic leukaemia is the most common leukemic cancer reported in children. Chemotherapy is the preferred treatment even though it continues to pose negative side effects of toxicity. Medicinal plants are reported to provide alternative treatment with lower toxicity levels. The three Amaryllidaceae species; Crinum bulbispermum, Boophone disticha, and Amaryllis belladonna Linnaeus have been reported for their anti-leukemic properties. These claims, however, lack supporting scientific data. The study aimed to investigate the anti-proliferative activity of the three Amaryllidaceae species against the human K562 leukaemia cells, as well as their phytochemical composition. The plants’ roots, bulbs and leaves were extracted with water, and sequentially with selected organic solvents. Cell antiproliferation was investigated using the SRB assay. Thin Layer Chromatography was performed to compare chemical profiles of different plant parts, and of plant samples collected fromdifferent geographic areas. Most plant parts tested positive for terpenoids and flavonoids. Only the bulbs contained phytosterols and alkaloids. Plant samples of C. bulbispermum obtained from two geographic areas had similar chemical profiles. Water bulb extract of C. bulbispermum and B. disticha showed over 70% cell growth inhibition at concentration of 10mg/ml, while their methanol extracts showed over 50% cell growth inhibition at 100mg/ml and 10mg/ml.Methanol root extract of A. belladonna L exhibited 100% cell growth inhibition at the concentration of 50mg/ml and over 80% at 25mg/ml concentration. In general, the polar extracts exhibited highest activity. The cell antiproliferation results obtained in this study support the use of the selected Amaryllidaceae species to treat leukemia as currently practiced in traditionalmedicine. The consistency of the constituents of the species, despite of their collection points, could enable standardization of traditionalmedicines. en_US
dc.language.iso en en_US
dc.publisher South African Journal of Botany Volume 135, December 2020, Pages 429-436 en_US
dc.relation.ispartofseries South African Journal of Botany;Volume 135, December 2020, Pages 429-436
dc.subject Acute lymphocytic leukaemia en_US
dc.subject Amaryllidaceae en_US
dc.subject Anti-proliferation en_US
dc.subject K562 cell line en_US
dc.subject Toxicity en_US
dc.title In Vitro Antiproliferative Activity Of Amaryllidaceae Species Against The K562 Human Leukaemia Cell Line en_US
dc.type Article en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


My Account