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Dacryodes Edulis (G. Don) H.J. Lam Modulates Glucose Metabolism, Cholinergic Activities And Nrf2 Expression, While Suppressing Oxidative Stress And Dyslipidemia In Diabetic Rats

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dc.contributor.author Erukainure, Ochuko, L.
dc.contributor.author Ijomone, Omamuyovwi, M.
dc.contributor.author Chukwuma, Chika, I.
dc.contributor.author Xiao, Xin.
dc.contributor.author Salau, Veronica, F.
dc.contributor.author Islam, Shahidul, M.D.
dc.date.accessioned 2023-04-20T07:12:13Z
dc.date.available 2023-04-20T07:12:13Z
dc.date.issued 2020-06
dc.identifier.citation Erukainure OL, Ijomone OM, Chukwuma CI, Xiao X, Salau VF, Islam MS. Dacryodes edulis (G. Don) H.J. Lam modulates glucose metabolism, cholinergic activities and Nrf2 expression, while suppressing oxidative stress and dyslipidemia in diabetic rats. J Ethnopharmacol. 2020 Jun 12;255:112744. doi: 10.1016/j.jep.2020.112744. Epub 2020 Mar 9. Erratum in: J Ethnopharmacol. 2021 Jan 30;265:113314. Erratum in: J Ethnopharmacol. 2022 May 10;289:115082. PMID: 32165174. en_US
dc.identifier.other 10.1016/j.jep.2020.112744
dc.identifier.uri http://hdl.handle.net/11462/2434
dc.description Article en_US
dc.description.abstract Ethnopharmacological relevance: Dacryodes edulis L. is an evergreen tree indigenous to western and eastern Africa which is utilized for nutritional and medicinal purposes. Folklorically, different parts of the tree are used in treating and managing diabetes and its complications. Aims: The antidiabetic effect of the butanol fraction of D. edulis ethanol extract (BFDE) was studied in fructosestreptozotocin induced type 2 diabetic rats. Methods: The ethanol extract was fractionated to yield the hexane, dichloromethane, ethyl acetate, butanol and aqueous fractions. The in vitro antidiabetic activities of the fractions were determined by their ability to inhibit α-glucosidase activity. BDFE was the most active and showed no cytotoxic effect while stimulating glucose uptake in 3T3-L1 adipocytes. Thus, selected for in vivo study. Diabetic rats were grouped into 4. The negative control group was administered water only, another group was treated with metformin (200 mg/kg bodyweight), while the other groups were administered BDFE at 150 and 300 mg/kg bodyweight respectively. Two other groups consisting of normal rats were given water and BFDE (300 mg/kg bodyweight) respectively, with the former serving as normal control. After 6 weeks of intervention, the rats were humanely sacrificed using appropriate anaesthesia. Results: Treatment with the fraction significantly (p < 0.05) reduced the blood glucose level of the diabetic rats, with concomitant increase in serum insulin secretion. It also caused significant (p < 0.05) elevation of reduced glutathione level, superoxide dismutase, catalase, α-amylase, and ATPase activities, with concomitant depletion in myeloperoxidase activity, NO and MDA levels of the serum and pancreas. The pancreatic morphology and β-cell function were significantly improved in BFDE-treated rats, with restoration of the pancreatic capillary networks. Treatment with BFDE significantly (p < 0.05) inhibited the activities of glycogen phosphorylase, fructose 1,6 biphosphatase, glucose 6 phosphatase, and acetylcholinesterase, while suppressing the expression of Nrf2. HPLC analysis revealed the presence of gallic acid, vanillic acid, vanillin, and (−)-epicatechin in the fraction. Conclusion: These results portray the antidiabetic and antioxidative properties of BFDE, which may be a synergistic consequence of the identified phenolics. en_US
dc.language.iso en en_US
dc.publisher Journal of Ethnopharmacology 255 (2020) 112744 en_US
dc.relation.ispartofseries Journal of Ethnopharmacology;255 (2020) 112744
dc.subject Antioxidant en_US
dc.subject Dacryodes edulis en_US
dc.subject Nrf2 en_US
dc.subject Phenolics en_US
dc.subject Type 2 diabetes en_US
dc.title Dacryodes Edulis (G. Don) H.J. Lam Modulates Glucose Metabolism, Cholinergic Activities And Nrf2 Expression, While Suppressing Oxidative Stress And Dyslipidemia In Diabetic Rats en_US
dc.type Article en_US

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