Abstract:
A total of 7364 ticks belonging to 13 species was collected from 64 game animals (belonging to 11 species)
and from 64 livestock animals (cattle and sheep) living in close vicinity at 6 localities in 3 South African
Provinces (Free State, Mpumalanga, and Limpopo). The geographic distribution of all tick species was
congruent with the literature except for Haemaphysalis silacea. From each infested host, a maximum
of 10 males and 10 females of each tick species were dissected to isolate the salivary glands. Salivary
glands were screened for tick-borne pathogens using polymerase chain reaction followed by reverse
line blotting and sequencing. This approach allowed us to evaluate the exposure of wild and domestic
hosts to tick-borne pathogens in their respective environments. Among the 2117 examined ticks, 329
(15.5%), belonging to 8 species, were infected and harboured 397 infections. Among those, 57.7% were
identified to species level and were assigned to 23 pathogen species of the genera Babesia, Theileria,
Anaplasma, and Ehrlichia. In 3 out of 6 localities, salivary glands from ticks infesting wild ruminants
displayed significantly higher infection prevalence and pathogen mean density than salivary glands from
ticks infesting livestock animals. Four piroplasm species [Theileria bicornis, Babesia sp. (sable), Theileria sp.
(giraffe), and Theileria sp. (kudu)] were detected for the first time in ticks. The tick species Rhipicephalus
evertsi evertsi, Rhipicephalus (Boophilus) decoloratus, Hyalomma rufipes, Rhipicephalus appendiculatus, and
Amblyomma hebraeum were associated with a broader pathogen range than previously known, and thus
new vector–pathogen combinations are described. In addition, previously unknown coinfection patterns
in tick salivary glands are reported.
