DSpace Repository

Cytochrome P450 monooxygenase analysis in free-living and symbiotic microalgae Coccomyxa sp. C-169 and Chlorella sp. NC64A

Show simple item record

dc.contributor.author Mthakathi, Ntsane Trevor
dc.contributor.author Kgosiemang, Ipeleng Kopano Rosinah
dc.contributor.author Chen, Wanping
dc.contributor.author Mohlatsane, Molikeng Eric
dc.contributor.author Mojahi, Thebeyapelo Jacob
dc.contributor.author Yu, Jae-Hyuk
dc.contributor.author Mashele, Samson Sitheni
dc.contributor.author Syed, Khajamohiddin
dc.date.accessioned 2019-01-21T13:23:01Z
dc.date.available 2019-01-21T13:23:01Z
dc.date.issued 2015
dc.identifier.issn 1226-2617
dc.identifier.issn 2093-0860
dc.identifier.uri http://hdl.handle.net/11462/1851
dc.description Published Article en_US
dc.description.abstract Microalgae research is gaining momentum because of their potential biotechnological applications, including the generation of biofuels. Genome sequencing analysis of two model microalgal species, polar free-living Coccomyxa sp. C-169 and symbiotic Chlorella sp. NC64A, revealed insights into the factors responsible for their lifestyle and unravelled biotechnologically valuable proteins. However, genome sequence analysis under-explored cytochrome P450 monooxygenases (P450s), heme-thiolate proteins ubiquitously present in species belonging to different biological kingdoms. In this study we performed genome data-mining, annotation and comparative analysis of P450s in these two model algal species. Sixty-nine P450s were found in two algal species. Coccomyxa sp. showed 40 P450s and Chlorella sp. showed 29 P450s in their genome. Sixty-eight P450s (>100 amino acid in length) were grouped into 32 P450 families and 46 P450 subfamilies. Among the P450 families, 27 P450 families were novel and not found in other biological kingdoms. The new P450 families are CYP745-CYP747, CYP845-CYP863, and CYP904-CYP908. Five P450 families, CYP51, CYP97, CYP710, CYP745, and CYP746, were commonly found between two algal species and 16 and 11 P450 families were unique to Coccomyxa sp. and Chlorella sp. Synteny analysis and gene-structure analysis revealed P450 duplications in both species. Functional analysis based on homolog P450s suggested that CYP51 and CYP710 family members are involved in membrane ergosterol biosynthesis. CYP55 and CYP97 family members are involved in nitric oxide reduction and biosynthesis of carotenoids. This is the first report on comparative analysis of P450s in the microalgal species Coccomyxa sp. C-169 and Chlorella sp. NC64A. en_US
dc.format.extent 610 381 bytes, 1 file
dc.format.mimetype Application/PDF
dc.language.iso en_US en_US
dc.publisher Algae en_US
dc.relation.ispartofseries Volume 30;Number 3
dc.subject carotenoid biosynthesis en_US
dc.subject Chlorella sp en_US
dc.subject NC64A en_US
dc.subject Coccomyxa sp. C-169 en_US
dc.subject cytochrome P450 monooxygenases en_US
dc.subject nitric oxide reductase en_US
dc.subject P450nor en_US
dc.subject symbiosis en_US
dc.title Cytochrome P450 monooxygenase analysis in free-living and symbiotic microalgae Coccomyxa sp. C-169 and Chlorella sp. NC64A en_US
dc.type Article en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


My Account