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Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?

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dc.contributor.author Smit, Francis Edwin
dc.contributor.author Bester, Dreyer
dc.contributor.author van den Heever, Johannes Jacobus
dc.contributor.author Schlegel, Franziska
dc.contributor.author Botes, Lezelle
dc.contributor.author Dohmen, Pascal Maria
dc.date.accessioned 2018-11-28T11:57:36Z
dc.date.available 2018-11-28T11:57:36Z
dc.date.issued 2015
dc.identifier.uri http://hdl.handle.net/11462/1809
dc.description Published Article en_US
dc.description.abstract This study investigated cryopreserved pulmonary homograft (CPA) structural integrity after prolonged cold ischemic harvesting times in a juvenile sheep model. Three groups with different post-mortem cold ischemic harvesting times were studied, i.e. Group 1 (24 h, n = 10); group 2 (48 h, n = 10); group 3 (72 h, n = 10). In each group, 5 CPAs were studied in vitro after cryopreservation and thawing. The other 5 CPAs were implanted in juvenile sheep for a minimum of 180 days. Serology samples were obtained and echocardiography was performed before euthanasia. Hematoxylin and eosin (H&E), scanning electron microscopy (SEM), von Kossa, Picrosirius red, α-actin, immunohistochemistry [von Willebrand factor (vWF), CD4, CD31 and CD34] and calcium content analyses were performed on explanted CPAs. The in vitro and in vivo studies failed to demonstrate any change in tensile strength, Young's Modulus and thermal denaturation (Td) results between the groups. SEM demonstrated a reduction in endothelial cells (50 % at 24 h, 60.9 % at 48 h and 40.9 % at 72 h), but H&E could not demonstrate autolysis in any CPA in vitro. All cultures were negative. In the explanted groups, IgE, IgM and IgG results were inconclusive. Echocardiography demonstrated normal valve function in all groups. H&E and Picrosirius red staining confirmed tissue integrity. vWF, CD31 and CD34 staining confirmed a monolayer of endothelial cells in all explanted valves. Calcium content of explanted CPA leaflets was similar. This experimental study supports the concept of prolonging the cold ischemic harvesting time of cryopreserved homografts to reduce homograft shortage. en_US
dc.format.extent 1 407 782 bytes, 1 file
dc.format.mimetype Application/PDF
dc.language.iso en_US en_US
dc.publisher Cell Tissue Bank en_US
dc.relation.ispartofseries Vol, 16;No, 4
dc.subject Cryopreserved homografts en_US
dc.subject Juvenile sheep model en_US
dc.subject Right ventricular outflow tract reconstruction en_US
dc.subject Warm ischemic time en_US
dc.title Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity? en_US
dc.type Article en_US

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