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Diversity and evolution of cytochrome P450 monooxygenases in Oomycetes

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dc.contributor.author Sello, Mopeli Marshal
dc.contributor.author Jafta, Norventia
dc.contributor.author Nelson, David R
dc.contributor.author Chen, Wanping
dc.contributor.author Yu, Jae-Hyuk
dc.contributor.author Parvez, Mohammad
dc.contributor.author Kgosiemang, Ipeleng Kopano Rosinah
dc.contributor.author Monyaki, Richie
dc.contributor.author Raselemane, Seiso Caiphus
dc.contributor.author Qhanya, Lehlohonolo Benedict
dc.contributor.author Mthakathi, Ntsane Trevor
dc.contributor.author Mashele, Samson Sitheni
dc.contributor.author Syed, Khajamohiddin
dc.date.accessioned 2018-11-28T11:47:55Z
dc.date.available 2018-11-28T11:47:55Z
dc.date.issued 2015
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/11462/1808
dc.description Published Article en_US
dc.description.abstract Cytochrome P450 monooxygenases (P450s) are heme-thiolate proteins whose role as drug targets against pathogens, as well as in valuable chemical production and bioremediation, has been explored. In this study we performed comprehensive comparative analysis of P450s in 13 newly explored oomycete pathogens. Three hundred and fifty-six P450s were found in oomycetes. These P450s were grouped into 15 P450 families and 84 P450 subfamilies. Among these, nine P450 families and 31 P450 subfamilies were newly found in oomycetes. Research revealed that oomycetes belonging to different orders contain distinct P450 families and subfamilies in their genomes. Evolutionary analysis and sequence homology data revealed P450 family blooms in oomycetes. Tandem arrangement of a large number of P450s belonging to the same family indicated that P450 family blooming is possibly due to its members’ duplications. A unique combination of amino acid patterns was observed at EXXR and CXG motifs for the P450 families CYP5014, CYP5015 and CYP5017. A novel P450 fusion protein (CYP5619 family) with an N-terminal P450 domain fused to a heme peroxidase/dioxygenase domain was discovered in Saprolegnia declina. Oomycete P450 patterns suggested host influence in shaping their P450 content. This manuscript serves as reference for future P450 annotations in newly explored oomycetes. en_US
dc.format.extent 2 734 547 bytes, 1 file
dc.format.mimetype Application/PDF
dc.language.iso en_US en_US
dc.publisher Springer Nature Limited: Scientific Reports en_US
dc.title Diversity and evolution of cytochrome P450 monooxygenases in Oomycetes en_US
dc.type Article en_US

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