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Comparative genomics of cytochrome P450 monooxygenases in newly explored pathogenic Oomycetes

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dc.contributor.author Mopeli, Marshal Sello
dc.date.accessioned 2018-07-09T08:53:23Z
dc.date.available 2018-07-09T08:53:23Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/11462/1415
dc.description Published Thesis en_US
dc.description.abstract Cytochrome P450 monooxygenases (P450s) are heme-thiolate proteins whose role as drug targets against pathogens, as well as in valuable chemical production and bioremediation, has been explored. In this study comprehensive comparative analysis of P450s in 13 newly explored oomycete pathogens performed. Three hundred and fifty-six P450s were identified in the 13 oomycetes species. These P450s were grouped into 15 P450 families and 84 P450 subfamilies. Among those, nine P450 families and 31 P450 subfamilies were newly identified in oomycetes. Research revealed that oomycetes belonging to different orders contain distinct P450 families and subfamilies in their genomes. Evolutionary analysis and sequence homology data revealed P450 family blooms in oomycetes. Tandem arrangement of a large number of P450s belonging to the same family suggested that, P450 family blooming is possibly due to duplications in family members. A unique combination of amino acid patterns was observed at EXXR and CXG motifs for the P450 families; CYP5014, CYP5015 and CYP5017. A novel P450 fusion protein (CYP5619 family) with an N-terminal P450 domain fused to a heme peroxidase/dioxygenase domain was discovered in Saprolegnia declina. Oomycetes P450 patterns suggested host influence in shaping their P450 content. This manuscript serves as reference for future P450 annotations in newly explored oomycetes. The study has been published in Scientific Reports (impact factor 5.6). Manuscript details: Sello, M. M., Jafta, N., Nelson, D. R., Chen, W., Yu, J., Parvez, M., Kgosiemang, I. K. R., Monyaki, R., Raselemane, S. C., Qhanya, L., Mthakathi, N. T., Mashele, S. S., Syed, K. (2015). Diversity and evolution of cytochrome P450 monooxygenases in Oomycetes. Scientific Reports, 5:11572. DOI: 10.1038/srep11572. Also the discovery of novel P450 protein was aired in South African TV channels (https://www.youtube.com/watch?v=VbOdUMTsEyc) and Newspapers. en_US
dc.format.extent 9 376 030 bytes, 1 file
dc.format.mimetype Application/PDF
dc.language.iso en_US en_US
dc.publisher Bloemfontein: Central University of Technology, Free State en_US
dc.title Comparative genomics of cytochrome P450 monooxygenases in newly explored pathogenic Oomycetes en_US
dc.type Thesis en_US
dc.rights.holder Central University of Technology, Free State

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